The main goal of PloidyNet is to train starting scientists in the field of chromosomal instability and aneuploidy, using a network of excellent participants throughout Europe. Through five scientific Work Packages we provide the best possible training, focusing on the causes and consequences of aneuploidy. Each participant contributes to multiple Work Packages and will at least spend a few months in another participant’s lab widening his/her horizon, further fostering network interactions.
The five work packages aim to train students in:
- The research of pathways that control chromosome segregation, in healthy and cancer cells.
- The approaches and technologies that are used to study the consequences of aneuploidy, focusing on four complementary methods: A reverse genetic screen, the development of p53 and p21 in vivo reporters, the in depth analysis of human aneuploid NSCLC samples, and the measurement of DNA damage on missegregated chromosomes.
- The use of mouse tumor models to study the contribution of aneuploidy and CIN to the initiation, development and progression of cancer
- How to drug mitotic pathways to best achieve efficient tumor cell death in vitro and in mice
- Developing biosensors to measure SAC activity, to develop tools to visualize chromosome segregation in vitro and in vivo, and to setup novel assays measuring aneuploidy fingerprint biomarkers using flow cytometry.
In addition, three more ‘non-scientific’ Work Packages were defined: Management, Training and Outreach, as these are three central activities within the network to which all participants will contribute. Our network collectively trains nine Early Stage Researchers and two Experienced Researchers. It will thus deliver a group of highly trained scientists, with a clear advantage for the next stage of their career.